Adam Simon

molecular biologist

Profile

Skilled molecular biologist with 6 six years of work experience obtained from different laboratories. Experienced with genetics related protocols and methods like DNA/RNA isolation and nucleic acid quantification. Extensive experience using RT-qPCR for research and diagnostic gained during my PhD research and from a COVID test laboratory. More than half a year of work record in a high throughput processing environment as a PCR Data Analyst.

Professional Experience

PCR Data Analyst

Enfer Group Unlimited, Naas

05/05/2020 - 28/11/2020

Applied quality control pipelines to ensure the integrity of RT-qPCR results from COVID-19 mass testing. Wrote PowerShell scripts to quickly retrieve certain data from qPCR generated text files. Wrote end of day reports summarizing positive, negative, invalid, and total tested samples. Handled special data retrieving requests from Data Analyst Lead and from Line Managers. Created and maintained SQL databases. Communicated between PCR and Data Analysis teams about failed plates. Assisted in writing SOPs. Trained new team members. Interacted with eLIMS: data upload, retrieval, report creation, identified and reported software issues.

Postdoctoral Researcher

National Institute for Bioprocessing Research and Training, Dublin

03/10/2019 - 31/03/2020

Programmed the hardware of a Liquid handling robot performed glycan sample preparation on it. Successfully glycoprofiled 581 individuals for immunoglobulin G with UPLC. Analyzed the results, with advanced statistics, identified biomarkers. Wrote SOPs for analysis workflow. Liaised with project partners and PIs. Wrote a research article.

Assistant Research Fellow

Laboratory of Bioseparation Sciences, University of Debrecen

14/12/2018 - 05/09/2019

Performed separation of glycans using capillary electrophoresis. Adapted machine learning multivariate statistics and applied on data. Created figures and wrote a manuscript. Identified glyco-biomarkers for myeloma diagnosis.

Education

PhD in Animal Science

University of Debrecen, Hungary

08/09/2014 - 14/12/2018 (graded Summa Cum Laude)

Thesis: Gene expression analysis of chicken under ad libitum and restricted feeding

Investigated the gene expression changes of peptide encoding genes in the chicken hypothalamus under different feeding states (fed-fasted-refed). Did basic research to identify potential feed intake regulator genes in order to further increase the genetic capacity of broiler chickens. Also investigated chicken small intestine proteomics changes during fasting. Published four articles in journals with impact factor regarding the topic. In addition to this, investigated the proteomics changes to performance traits in chicken and sheep, too.

MSc in Molecular Biology

University of Debrecen, Hungary

09/09/2012 - 14/06/2014 (graded excellent, 96.2%)

Thesis: Detection of cattle melanocortin-1 receptor gene SNPs with PCR-SSCP method

BSc in Biology

University of Debrecen, Hungary

09/09/2008 - 16/06/2011 (graded good, 80%)

Thesis: Micropropagation of an endangered Crocus species from the Carpathian basin

Digital Skills

  • MS Office 2019
  • Illustrator
  • GraphPad
  • Photoshop
  • SPSS
  • RStudio
  • PowerShell
  • LinRegPCR
  • GlycoWorkbench
  • HTML/CSS
  • Oligo 7
  • ImageJ

Competence in Laboratory Techniques

Able to program and perform sample preparation with liquid handling robots

DNA, RNA and protein isolation from different matrices

Immunohistochemistry, creating tissue dissections, antibody labelling, fluorescent microscopy

Inducing and maintaining plant cell cultures, creating media stocks, ability to work under aseptic conditions

Proteomics using two-dimensional difference gel electrophoresis (2D-DIGE)

Quantification of proteins (using ELISA and WB)

Relative quantification of nucleic acids using quantitative real-time polymerase chain reaction (qPCR)

Separation of nucleic acid with capillary electrophoresis (CE)

Ultra-Performance Liquid Chromatography (UPLC) separation of N-glycans

Licenses and Certifications

FELASA accredited certificate (level B) in Laboratory Animal Science and Welfare (2016)

Radioisotope handling certificate (2015)

Full driver’s license (2009)

Courses and Workshops

Advanced Time Management skills - NIBRT (2020)

HPLC course - Budapest University of Technology and Economics (2019)

RStudio for biostatistics - Software Carpentry Workshop, University of Debrecen (2018)

Summer School of poultry health and nutrition - University of Veterinary Medicine, Vienna (2018)

Proteomics workshop - University of Debrecen (2017)

Lecturing / Tutoring Experience

Demonstrated practical laboratory skills of Molecular Genetics and Proteomics for Food Science MSc and Biotechnology MSc students

Ensured students followed health and safety guidelines

Explained theoretical reasoning behind practical skills and protocols

Supervised the laboratory part of BSc students theis work

Have given lectures outside academia on science popularizing eventss

Administrative Responsibilities

Person responsible for managing laboratory consumables in different research groups, ensured that the chemicals/plasticware are compatible with the instruments, ordered on time and within the budget

Person responsible for selecting and ordering laboratory equipment and instruments for animal science projects

Writing ethical approvals for animal experiments

Honours and Awards

Commemorative Medal, Contribution to National Covid-19 testing - Enfer Labs (2020)

Predoctoral Award - National Excellence Programme (2017)

Students’ Scientific Association Award for MSc thesis (2013)

Undergrad Student Award for BSs thesis - Universitas Foundation (2011)

Volunteer Experience

Regular blood plasma donation - Plasmapheresis station at the University of Debrecen, Faculty of Medicine (2009-2014)

Recent Publications

Simon A, O’Flaherty R, Gonzalez-Quintela A, Gude F, Fahey R. Serum N-glycans can aid the classical biomarkers to differentiate between abstainers and heavy drinkers. 2020. In preparation.

Gebri E, Kovacs Z, Meszaros B, Toth F, Simon A, Jankovics H, Vonderviszt F, Kiss A, Guttman A, Hortobagyi T. N-Glycosylation Alteration of Serum and Salivary Immunoglobulin a Is a Possible Biomarker in Oral Mucositis. Journal of Clinical Medicine. 2020;9(6):1747.

Simon A, Gulyas G, Meszar Z, Bhide M, Olah J, Bai P, Csosz E, Javor A, Komlosi I, Remenyik J, Czegledi L. Proteomics alterations in chicken jejunum caused by 24 h fasting. PeerJ. 2019;7:e6588.

Simon A, Nemeth J, Javor A, Komlosi I, Bai P, Olah J, Juhasz B, Kiss R, Szilvassy Z, Czegledi L. Feeding state and age dependent changes in melanin-concentrating hormone expression in the hypothalamus of broiler chickens. Acta Biochim Pol. 2018;65(2):251-8.

Simon A, Javor A, Bai P, Olah J, Czegledi L. Reference gene selection for reverse transcription quantitative polymerase chain reaction in chicken hypothalamus under different feeding status. J Anim Physiol Anim Nutr. 2018;102(1):286-96.

Referees

Are available on request